For Methodology , Two soy-yoghurt premixes were formulated to contain: (i) soy milk (ii) soy milk plus nunu (50:50). Mixtures of premixes, sugar 3% and gelatin 0.5% were prepared, homogenized and pasteurized . Take it in a water bath to cool down to 43°C .
The first portion was inoculated with 1% commercial yoghurt culture (50:50) mixture of Lactobacillus bulgaricus and Streptococcus thermophilus .
the second portion was inoculated A preculture of each of the two LAB isolates from nunu (Lactobacillus bulgaricus and Streptococcus thermophilus) and the mixed culture inoculated into the second portion of cooled mixture of each of the premixes at 5% final volume as described.
All the inoculated premixes were poured into plastic cups and incubated at 43°C to ferment for 12 hours. After incubation, the premixes were cooled in an ice bath, placed in a cabinet at 6±2°C until evaluation within 12 hours.