In addition, etiolated and
non-etiolated petioles were excised from the tubers and cultured
on media with the same composition as for callus culture. Callus
formed on all explant types. Peduncle and petal explants on
medium containing 0.22 mg l−1 TDZ exhibited the most callus
growth after 8 weeks of culture. Callus formed on both etiolated and
non-etiolated explants, but callus growth was significantly greater
on etiolated than on non-etiolated explants after 8 weeks of culture,
which is in agreement with the observations of Ando and Murasaki
(1983).
In our