Materials & Methods:
Sample collection: Sewage water samples were
collected in the sterilized sampling bottles for isolation
of E. coli from different locations of People’s Campus,
Bhanpur, Bhopal (M.P).
Isolation of bacterial cultures: In order to isolate
the E. coli from sewage samples, serial dilution method
was used (Waksman & Reilly, 1945). Dilution (10-3)
was streaked on the surface of sterilized eosin
methylene blue agar (EMB) plates and incubated at
37°C for 24 hours. The colonies possessing green
metallic sheen (characteristic of E. coli on EMB
medium) were isolated, purified and preserved on
Sabouraud’s dextrose agar (SDA) slants.