2.7. Laser confocal micro-Raman (LCM-Raman) spectroscopy
A Renishaw Invia Raman microscope system (Renishaw, Gloucestershire,
United Kingdom) equipped with a Leica microscope
(Leica Biosystems, Wetzlar, Germany) and a 785 nm green diode
laser source was used in this study. Spectra were taken from the
same spot size of each sample in the range of 3200e100 cm1 with
a resolution of approximately 7 cm1
. The full width at half
maximum (FWHM) of the band at 480 cm1 was calculated by
using the software of WiRE 2.0.
2