The enzyme assay was carried out by two methods CMCase and FPase and the production was analyzed individually by Miller‘s modified method of Dinitrosalicylic acid (DNSA). In this study the high
level of enzyme production was obtained at 300C and 400C by using Rice husks, Banana peels, Millet husks, Wheat bran, saw dust and Coir waste by using varying substrate concentrations. Out of all the six used substrates banana peels gave the higher production of the cellulase enzyme that is 12.4 units / ml after 4 days of incubation.