I. Introduction
Materials and Methods
Isolation and Identification of E. coli Each sample was homogenized in a sterile electrical blender and 1 gram of the homogenized sample was suspended in 9 ml of sterile physiological saline (0.85% NaCl). Serial dilutions up to 10 -7were made and 1 ml of each dilution was plated on both Eosin Methylene Blue (EMB) and Mac Conkey agars. The plates were incubated at 37 Cº for 24 hours. Colonies exhibited metallic sheen on EMB agar and pink color on Mac Conkey agar were subcultured to obtain pure culture. Pure cultures were tested biochemically using API 20 E strips (Bio Merioux, France) to confirm as E. coli