The results exhibit that suspension of cell culture contains viable mammalian cells 10-20 microns, non-viable cells 5-10 microns, and cell debris that are less than 5 microns. The suspension is separated in a pilot disk-stack centrifuge with a bowl diameter of about 190 mm. The cell debris and nonviable cells are removed in the centrate, and the viable cells are captured in the concentrate. The model presented in the chapter is used to simulate conditions that may not have experimental results or extend to conditions where experimental results are limited or lacking.