B. cinerea spores were sensitive to the chitosan film incorporating 0.1% GSE or 0.5% GSE alone. More than 60% of the spores germinated in PDA plates after a 60 s immersion in 1% chitosan alone. The combination of 0.1% GSE and 1% chitosan achieved a significant result, reducing the spore germination rate to only 25%, indicating an apparent combined antifungal effect of chi- tosan and GSE.