Micropropagation
The explants were inoculated in solidified MS
medium supplemented with different
concentrations of growth regulators i.e. BAP,
Kinetin and 2,4-D in initial media. After
inoculation of the explants in culture bottles and
tubes, the culture bottles and tubes were kept
25 ± 2 ºC for 2-3 weeks. After 2-3 weeks of
duration of inoculation, the healthy and noninfected
explants were transferred to callus
inducing media with the same hormonal
compositions. The cultures were again
incubated in culture room at 25 ± 2ºC for 2-3
weeks. The culture in which callusing was established and the plants were observed as
acclimatized.