2.7.1. Membrane packing followed by MC540
Liposomes formulations (before and after pasteurization) with a 50 mM concentration were diluted with distilled water until they reach a 0.868 mM concentration. Probe concentration was 4.344 103 mM, incorporated into the vesicles with a probe/lipid ratio of 1/200 (Bernik and Disalvo, 1993).A scan of each sample between 400 and 600 nm was obtained with a UV–VIS spectrophotometer (Shimadzu). Measurements were performed at room temperature and baseline correction was done as previously described (Bernik and Disalvo, 1996).Partition coefficient (PC) was calculated as: PC = A570/A530,
this parameter relates concentration of monomer in non-polar environment with respect to monomer in an aqueous phase