This study was carried out to evaluate the effect of heating prior to drying and storage temperature on the antioxidant activity of turmeric (Curcuma longa L.). The antioxidant activity of its crude extract was carried out using DPPH radical scavenging activity. The result showed that EC50 values of the crude extracts from turmeric prepared from heating prior to drying were higher than those of unheated sample and unheated commercial powder. The effect of storage temperatures (30 and 40°C) on the EC50 values of crude extract from turmeric powder with heating prior to drying and the commercial one was investigated, the results revealed that higher storage temperature reduced the antioxidant activity (higher EC50) of both samples. In addition, during the storage EC50 values of crude extract from turmeric powder heated prior to drying were lower than extracts from commercially available turmeric powder. These results suggest that heating turmeric prior drying and keeping at low temperature the antioxidant activity of turmeric powder.