2.5. Binding of Tbz
1 mM of standard solution of thiabendazole was prepared by dissolving
in a solvent mixture of 20 mM × Tris–HCl (pH 7.4)-DMF
(90%–10%, v/v). Excitation and emission wavelengths of the drug
were scanned by fluorescence spectroscopy. Drug solution was
added to the appropriate amount of Hb immobilized GPTS–SPIONs in
20 mM × Tris–HCl (pH 7.4). Each mixture was mixed for 30 min at a
particle concentration range of 2.5–25 mg mL−1 [13]. The nanoparticles
were separated using a 0.5 T magnet. Emission spectra of the remaining
solution were recorded at 299 nm and 358 nm of excitation and emission
wavelengths. The binding amount of the drug was determined
from the regression equation of the calibration curve.