Serum glucose was analyzed using the automated
procedure of Gochman and Schmitz ( 19721, and
serum lactate concentrations were measured in a
Model 23L Lactate Analyzer (Yellow Springs Instrument,
Yellow Springs, OH) following the procedure
outlined by Apple et al. (1993b). Serum free fatty acid
concentrations were determined optically using a
commercially available enzymatic kit (Wako Chemicals
USA, Dallas, TX).
Not all lambs are representeda t all sampling times
in the results for blood endocrine and metabolite
analyses because of temporarily blocked catheters,
erroneous assay readings, or other reasons. The
number of lambs represented is stated with each
figure.