Research was conducted, to develop an in vitro technique for short-term conservation of banana, cv. Dwarf Cavendish during the period 1997-1998. In this study, minimum growth conservation has been attempted with 2 temperature regimes (22 0C and 15 0 C) and 3 levels of MS medium concentrations (1 MS, ½ MS and ¼MS) using shoot-tip cultures of Dwarf Cavendish banana. The survival percentage of shoot-tip explants decreased significantly with the increase in incubation time. The survival percentage of shoot-tips explants decreased significantly with the increase in incubation time. The best temperature for in vitro conservation was 15 0C and the best medium concentration was ½ MS. Temperature, MS medium concentration and interaction between conservation time and concentration of MS medium had no significant effect on number of both shoots and roots of banana cv. Dwarf Cavendish. Interaction between temperature and MS medium concentration significantly influenced number of roots.