Results of glucoamylase activities obtained from crude culture filtrates of Aspergillus niger and Rhizopus species obtained as outlined in section 3.4.2 on wheat bran are presented in appendix 18. Crude culture filtrate of Aspergillus niger produced at 40ºC had the highest glucoamylase activity of 3.50 U/ml in 48 hours. Crude culture filtrate of Rhizopus species produced at 40ºC had glucoamylase activity of 3.00 U/ml in 48 hours. In this work, the optimum temperature for glucocoamylase production by both organisms was 40ºC (Figure 4.6).