2.2. Micro-emulsions preparation
The laccase micro-emulsions were prepared by contact of an organic (vegetable oil: Oleo Fula–food grade from Sovena, SA) and an aqueous phase (protein, bovine serum albumin, in acetate buffer pH5).
Micro-emulsions in a ratio of 0.5 oil/99.5 water were prepared using different concentrations of BSA (2.5; 5 and 10 g/L) and of lac-case (1.5; 2.5; 5 and 10 g/L) to form an oil-in-water (O/W) emulsion.The two phases once mixed were homogenized (39 cycles—13 min)using a homogenizer (EmulsiFlex-C3—Avestin, Canada) under high pressure (1000 bar).