Hardening is a slow and controlled processes of exposing or habituating the in vitro grown plantlets to outside environment.
Hardening of rooted plants was 100% in coco peat.
Hardening for 2 weeks under reduced evapotranspiration losses improved the survival percentage (Table 3).
Inverted jam bottles or transparent plastic cups when used to cover the rooted plants during hardening phase improved the initial survival in coco peat.
Hardening conditions for A. auriculiformis were not reported by earlier (Mittal et al., 1989; Zhang et al., 1995).