Subsequently, microorganisms were separated
from the culture media by centrifugation at 4000 ×g for 30 min at
4 °C. The precipitate was resuspended in sterile saline water with 3%
NaCl (Synth®, Diadema, SP) and the concentration was adjusted to
104 CFU·mL−1 with previously constructed standard curve (OD
630 nm). After preparation of the solutions, they were added at a concentration
of 104 CFU·mL−1 in post-larval culture tanks. The microbiological
parameters of the culture water in bioflakes were analyzed.