2.3. PB lime requirement method
1. Prepare themodified AE buffer solution by dissolving 10 g of p-nitrophenol,
37 g potassium chloride, 7.5 g boric acid (H3BO3), and 5.25 g
potassium hydroxide (KOH) in 1,000 mL distilled water and
adjusting pH to 8.00 ± 0.10 if necessary (Pillai and Boyd, 1985). A
pH change of 0.1 unit in the buffer equals 0.16 mEq acidity.
2. Weigh 20.0 g of dry, pulverized pond soil, put into a 125-mL Erlenmeyer
flask, add 40 mL buffer solution, and place on a mechanical
shaker (200 rpm) for 30 min.
3. Measure the pH of the soil–buffer mixture with a glass electrode.
4. Calculate the lime requirement by the equation:
2.3. PB lime requirement method1. Prepare themodified AE buffer solution by dissolving 10 g of p-nitrophenol,37 g potassium chloride, 7.5 g boric acid (H3BO3), and 5.25 gpotassium hydroxide (KOH) in 1,000 mL distilled water andadjusting pH to 8.00 ± 0.10 if necessary (Pillai and Boyd, 1985). ApH change of 0.1 unit in the buffer equals 0.16 mEq acidity.2. Weigh 20.0 g of dry, pulverized pond soil, put into a 125-mL Erlenmeyerflask, add 40 mL buffer solution, and place on a mechanicalshaker (200 rpm) for 30 min.3. Measure the pH of the soil–buffer mixture with a glass electrode.4. Calculate the lime requirement by the equation:
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