PREPARATION OF STANDARD SOLUTIONS
HPLC method
Twenty five mg of accurately weighed standard
fluconazole was dissolved and made up to mark with
water, in a 50 ml volumetric flask, to get primary stock
solution of 500 μg/ml. Serial dilutions were made to
obtain 1, 2, 4, 8, 20, 40 and 100 μg/ml using mobile
phase. All solutions were filtered through 0.45 μ
membrane filter prior to use.