Rice bran hydrolysate consisted of 18 g/L glucose, furfural 198.68 ppm, 5-hydroxymethyl furfural (5-HMF) 0.095 ppm, vanillin 0.86 ppm, and syringaldehyde 3.19 ppm, and 0.168% acetic acid was used as a substrate for an ethanol production in this study.