2.2.2. GABA determination
For the GABA extraction, an aliquot of the wheat flour dough
medium was centrifuged at 20,000 g for 15 min at 4 C. GABA was
quantified by Reversed-Phase High-Performance Liquid Chromatography,
followed by UV detection after precolumn derivatization
by the reagent methodology, 6-aminoquinolyl-N-hydroxy succinyl
carbamate (AccQTag) (Cohen & Michaud, 1993).