The primers TP1 and TP2 were used to amplify kanamycin
resistance gene by PCR. The EcoRI, NcoI, BamHI and
XcmI sites were introduced at upstream of kanamycin
resistance gene and the opposite XcmI site was designated
at downstream of kanamycin resistance gene. It
was named as the XcmI cassette. After PCR amplification,
agarose electrophoresis showed a band of approximately
900 bp consistent with fragment of kanamycin
resistance gene.