The aim of this study was to assess the synchrony in follicular wave emergence and subse-quent ovulation following dominant follicle ablation or estradiol-17 administration. Sixcycling Murrah buffaloes were sequentially allotted to three groups, that is, control, follic-ular ablation, and estradiol-17 groups. For the control group, buffaloes at random stagesof estrous cycle were examined daily by transrectal ultrasonography for 14 days and theday of wave emergence was recorded. Following induced luteolysis and ovulation (Day 0),these buffaloes were included in the ablation group. All follicles (>5 mm) were ablated onDay 3 or 5 or 7 (n = 2 each day). Seven days after the ablation, these buffaloes were admin-istered prostaglandin F2to induce luteolysis and ovulation. Following this, buffaloes wereincluded in the estradiol treatment group with estradiol administered on similar days asfor ablation in the ablation group. Luteolysis was induced nine days after the estradiolinjection. All animals of the treatment groups were subjected to transrectal ultrasound andblood samplings daily from treatment to induced ovulation. The follicular waves emergedsignificantly earlier (P = 0.001) in both the ablation (2.1 ± 0.79 days) and estradiol (4.0 ± 0.25days) treatment groups than the control group (8.3 ± 0.88 days). The deviation from meanday of ovulation was greater (P = 0.02) for the control group buffaloes (1.66 ± 0.3 day) thanthose of the treatment groups (ablation, 0.76 ± 0.2 and estradiol, 0.58 ± 0.2 day). In con-clusion, both ablation and estradiol resulted in synchronous emergence of a new follicularwave irrespective of stage at which the treatment was given, with greater synchrony ofovulations in water buffalo.