It was then cloned into the pCR2.1 vector (Invitrogen, Carlsbad, CA, USA)
which was used to transform Top10 competent cell. The insert was
bidirectionally sequenced using universal M13 primers (Macrogen,
Daejon, Korea). DNA sequences were analyzed using the DNAstar
program (Version 5.01, DNAstar Inc., Madison, USA). The resulting
insert sequences were tested in their homologies with known mtDNAs
using the BLAST program