The results showed that the highest amount of Monacolin K in crude extract was found in extraction using 95% v/v of ethanol (MFR95) whereas effect of heating procedure also showed that the amount of Monacolin K in crude extract was increased in 60 °C. HPLC analysis showed that the yield of Monacolin K in crude of MFR95 extract was 1760 μg g-1. In conclusion, ethanol 95% v/v and heated in 60 °C were the best choice for extraction of Monacolin K from MFR.