The aim of thisstudy was to evaluate the in vitro fermentability of scFOS by ileal bacteria and the effectof scFOS on concentrations of the total anaerobic bacteria, Lactobacilli, Streptococci, Bifidobacteriaand Clostridium perfringens in veal calf ileal contents. Two groups of 4 calves fed afinisher milk replacer and fibrous pellets were sacrificed 5 h after the morning meal. In eachgroup, ileal contents were pooled and diluted with Lowe’s medium. Two amounts of scFOS(100 and 250 mg) were tested in vitro versus a control (0mg scFOS). Each bottle was incubatedin duplicate for each treatment and each time point at 37 ◦C in a shaking water bath.Bacterial concentrations were determined at 0, 3, 6, and 9 h and pH, gas production, volatilefatty acids (VFA), and lactate concentrations were measured every 1.5 h for 9 h. Over time,scFOS fermentation resulted in more gas production (P=0.055), higher total VFA concentrations(P<0.01), an increase in l-lactate concentration (P<0.01), as well as a lower pH value(P<0.01) than the control bottle. Acetate and butyrate concentrations increased (P<0.01)with scFOS fermentation. ScFOS fermentation enhanced growth of Lactobacilli (P=0.010),Streptococci (P=0.015) and lactic acid utilizing bacteria (P=0.025). C. perfringens concentrationwas not affected by scFOS addition. Based on our results, scFOS was fermented bybacteria of ileal contents in veal calves and its fermentation led to a selective stimulationof host bacteria. However, further studies are needed to determine impacts of scFOS on C.perfringens concentration in veal calf ileal contents.
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