Genomic DNA from L. plantarumWCFS1 was extracted. The gene
encoding a putative lipase/esterase (lp_3562) in L. plantarum
WCFS1 was amplified by PCR by using the primers 575 (50-
GGTGAAAACCTGTATTTCCAGGGCatggagatacgcacactagctttaa) and 576
(50-ATCGATAAGCTTAGTTAGCTATTACTAaatttcagtactattcttgcta) (the
nucleotides pairing the expression vector sequence are indicated in
italics, and the nucleotides pairing the lp_3562 gene sequence are
written in lowercase letters).