Lavender (Lavandula Multifida) is in a family Lamiaceae. Most lavender originates in the Mediterranean basin, in rocky, calcareous areas. Lavender occurs over North Africa, the Mediterranean, Europe and Western India (Department of Agriculture, Forestry and Fisheries, 2009). The stems are gray and wooly. Leaves are double pinnate and blue violet flowers. Products of lavender are essential oil and flower (Beckett, 1995). Flower and smaller quantities of leaves are used for oil distillation. Lavender is sensitive with high temperature, but it can tolerate frost moderately. In natural condition, true lavender grows at average 1,700 m above sea level, while spike lavender grows only at lower altitude, and is native in range of 200 to 700 m above sea level. In mild climate, autumn is better to grow plants than in winter. However, it grows quickies in spring. In area with very cold winter, spring planting is the only option. Planting should be started as soon as danger of frost is passed (Department of Agriculture, Forestry and Fisheries, 2009). The highland of Northern Thailand has a potential to grow lavender but little is known about suitable temperature for lavender cultivation. Therefore this study was aimed to determine the effect of temperature on growth and development of Lavendula multifida.