4.3.1.2.2. Antibody-based antigen detection methods (IFAT) without cell culture
Samples to be tested include stamp-smear of spleen from affected fish.
Use a stamp-smear of spleen from uninfected fish as a negative control, and use a stamp-smear of
spleen from confirmed RSIV-infected fish as a positive control.
Positive controls (air-dried and fixed stamp-smear of spleen from infected fish) can be obtained from
the OIE Reference Laboratory. Use imprints of healthy fish spleen as negative controls.