Experiments were performed in triplicate. Data were presented
as means ± standard deviation and the differences between groups
were considered significant at p < 0.05. The analysis of variance
(ANOVA) was used to evaluate the significance in the difference
between factors and levels. Comparison of the means was done by
Dunnet test. The statistical analysis of the data was performed using
GraphPad InStat software (GraphPad Software Inc., La Jolla,
USA).