③ Ohmic heating
1. Ohmic heating is a novel method to deliver thermal energy to the heating medium
- To process food particulates aseptically
- Maximize gel formation of Pacific whiting surimi,
- Remove high-molecular-weight proteins in surimi wash water (SWW)
- To recover heat-stable cathepsins from SWW
2. The process served as a good preliminary method to remove contaminating proteins, mainly of
myofibrillar proteins
3. Ohmic heating recovered higher total activity (193%) of the cathepsin
6) Alternative methods for enzyme recovery
① Precipitation
1. The recovery of enzymes can be facilitated by selective denaturation of contaminating proteins
2. The simplest way to achieve selective denaturation is by careful heating
3. Shifting the pH of the homogenate can also regarded as a very simple process
4. To achieve optimum purification effects, both methods can be combined
- By using commercial baker’s yeast, 100% recovery of invertase with the 73 purification factor
combining two steps:
⑴ Acidification
⑵ Heat treatment