Preparation of germinated brown rice by soaking and anaerobic incubation
Red aromatic brown rice was sterilized using 0.1% sodium hypochlorite for 0.5 h and rinsed with tap water. Then brown rice was soaked in sterile water (1:3 w/v) at different temperatures (5, 28, 35oC) and times (8, 16 and 24 h) with the water being changed every 6 h. After soaking, the grains were placed on moist filter paper in covered glass dishes and kept at room temperature in the dark for 24 h. Germinated brown rice was dried with a tray dryer at 50oC until moisture content was less than 13%. Dried germinated brown rice was then ground, sieved through a 100 mesh screen and kept at 4oC for GABA analysis. The soaking condition that gave the highest GABA content was selected for further study.