The experimental design was a randomized complete
block design with 4 treatments; room was considered
the experimental unit. Rooms used were switched
within trials to remove room effects. Two trials were
repeated over time where trial serves as the blocking
factor with 4 replicates/trial. Analyses were conducted
using PROC MIXED (SAS Institute, 2010). Means
comparisons on d 21, 28, 42, and 56 were assessed
by least-significant differences test, and statements of
significance were based on P ≤ 0.05, unless otherwise
stated. ANOVA combined across days were performed
to obtain treatment comparisons averaged across days
with equal variances between days.