For the analysis of individual phenolics by targeted HPLC and
untargeted LC–QTOF–MS based measurements, 0.4 g freeze-dried
sample was extracted with 3.0 mL 75% methanol in MQ water acidified
with 0.1% formic acid. Extracts were sonicated for 15 min,
centrifuged at 2500 rpm for 10 min and filtered through 0.45 lm
filters (Minisart SRP4, Biotech GmbH, Germany) (Capanoglu et al.,
2008).