Onehundred milligrams of the dry extracted lipids was separated using
NH2-aminopropyl minicolumns, following the method of Kaluzny,
Duncan, Merritt, and Epps (1985). Neutral lipids (NL) were eluted
with 3.0 ml of CHCl3: iso-proponal (2:1), free fatty acids (FFAs) were
eluted with 3.0 ml of diethyl ether: acetic acid 2%, and phospholipids
(PL) were eluted with 3.0 ml of MeOH. The amounts of NL,
FFAs and PL were expressed as g/100 g total lipids obtained.