an extended prepulse to fully inactivate the current, then repolarizing
the cell and measuring the current elicited by a test
pulse after a certain delay. In this manner, recovery could be
distinguished from phenomena such as facilitation or inactivation
from intermediate closed states. The initial experiments
investigated the time course of recovery after repolarization to
3110 mV. As other studies have shown [15,16], recovery of
the calcium current is best ¢t by a biexponential function. The
parameters of curves ¢t to individual experiments varied signi
¢cantly, so curves were ¢t to mean values to provide an
estimate of the fast and slow time constants of recovery.
The faster component accounted for 40% of the recovery of
the current carried by cyCav1 (Fig. 2A and Table 1). Coexpression
of any L subunit accelerated the recovery of cyCav1,
though the degree of potentiation di¡ered for each subunit.
cyL had the greatest e¡ect, followed by L4 and L3, each of
which decreased the time constants of both components of
recovery. The cyL subunit also increased the proportion of
recovery accounted for by the fast component, as did the
an extended prepulse to fully inactivate the current, then repolarizingthe cell and measuring the current elicited by a testpulse after a certain delay. In this manner, recovery could bedistinguished from phenomena such as facilitation or inactivationfrom intermediate closed states. The initial experimentsinvestigated the time course of recovery after repolarization to3110 mV. As other studies have shown [15,16], recovery ofthe calcium current is best ¢t by a biexponential function. Theparameters of curves ¢t to individual experiments varied signi¢cantly, so curves were ¢t to mean values to provide anestimate of the fast and slow time constants of recovery.The faster component accounted for 40% of the recovery ofthe current carried by cyCav1 (Fig. 2A and Table 1). Coexpressionof any L subunit accelerated the recovery of cyCav1,though the degree of potentiation di¡ered for each subunit.cyL had the greatest e¡ect, followed by L4 and L3, each ofwhich decreased the time constants of both components ofrecovery. The cyL subunit also increased the proportion ofrecovery accounted for by the fast component, as did the
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