The two-week-cryopreserved sperm samples in the four extenders were thawed in warm water (40oC) for one minute and stained with eosin-negrosin dye (Merck) for a determination of the percentage of both living and dead sperm. .
The two-week-cryopreserved sperm samples in the four extenders were thawed in warm water (40oC) for one minute and stained with eosin-negrosin dye (Merck) for a determination of the percentage of both living and dead sperm..