2.3. Study design
The present work was specifically designed to study the effects of freezing and modified atmosphere packaging (MAP) (100% N2 and 50% N2–50% CO2) compared to vacuum packaging and conventional method of sulphite (SUL) treatment on the melanosis and selected chemical qualities of Giant Red Shrimp (GRS) (A. foliacea) during 12-month period. Choosing the abovementioned was necessitated by our quest for an alternative to the chemical approach of sulphite treatment commonly/traditionally used by the fishery sector. In this context, the commonly/traditionally used sulphite treatment performs the function of control because it is the basis of comparison to the other alternative treatments. Well thought through also is the choice of adapting 100% N2 and 50% N2 + 50% CO2, which emanated from the biochemical reactions that foundationally underpin the enzymatic production actuating the formation of black spot of shrimp. In fact, it is to authors’ comprehension that given the important role oxygen plays in this reaction, removing the oxygen from this reaction and replacing it on one hand with only inert gas, and on the other, with gas mixture containing inert + another acidifying-enhancing gas, would cumulatively demonstrate improved ‘food technovation’. For emphasis, the investigated characteristic qualities involved proximal and gas compositions, melanosis scoring, chemical parameters of pH, TVB, TBA as well as FAAs. A total of 40 kg of shrimp that were divided into four lots sufficed for the study. Each lot apportioned equally into barrier bags that contained about 800 g of GRS samples. Immediately on-board, GRS samples were quickly processed. Specifically, whereas prior to packaging 100% N2 MAP and vacuum packaged samples was subject to blast freezing (−35 °C), samples of 50% N2 + 50% CO2 MAP underwent treatment prior to blast freezing. This approach was selected to allow for CO2 dissolution at water phase (Devlieghere, Debevere, & Van Impe, 1998) of the GRS flesh. Essentially, experimental/laboratory analyses commenced from day 3 after harvest of GRS samples. Prior to all analytical tests, the shrimp were beheaded, peeled and deveined. In most cases, experimental/laboratory analyses were regularly implemented every two months during the 12-month period, which afforded between 5 and 7 data points of outcomes of storage measurements of tested parameters against storage time. Unless otherwise indicated, analyses were repeated three times and independently per treatment using different GRS samples. All chemicals and reagents employed are of analytical grade.
2.3. ศึกษาการออกแบบThe present work was specifically designed to study the effects of freezing and modified atmosphere packaging (MAP) (100% N2 and 50% N2–50% CO2) compared to vacuum packaging and conventional method of sulphite (SUL) treatment on the melanosis and selected chemical qualities of Giant Red Shrimp (GRS) (A. foliacea) during 12-month period. Choosing the abovementioned was necessitated by our quest for an alternative to the chemical approach of sulphite treatment commonly/traditionally used by the fishery sector. In this context, the commonly/traditionally used sulphite treatment performs the function of control because it is the basis of comparison to the other alternative treatments. Well thought through also is the choice of adapting 100% N2 and 50% N2 + 50% CO2, which emanated from the biochemical reactions that foundationally underpin the enzymatic production actuating the formation of black spot of shrimp. In fact, it is to authors’ comprehension that given the important role oxygen plays in this reaction, removing the oxygen from this reaction and replacing it on one hand with only inert gas, and on the other, with gas mixture containing inert + another acidifying-enhancing gas, would cumulatively demonstrate improved ‘food technovation’. For emphasis, the investigated characteristic qualities involved proximal and gas compositions, melanosis scoring, chemical parameters of pH, TVB, TBA as well as FAAs. A total of 40 kg of shrimp that were divided into four lots sufficed for the study. Each lot apportioned equally into barrier bags that contained about 800 g of GRS samples. Immediately on-board, GRS samples were quickly processed. Specifically, whereas prior to packaging 100% N2 MAP and vacuum packaged samples was subject to blast freezing (−35 °C), samples of 50% N2 + 50% CO2 MAP underwent treatment prior to blast freezing. This approach was selected to allow for CO2 dissolution at water phase (Devlieghere, Debevere, & Van Impe, 1998) of the GRS flesh. Essentially, experimental/laboratory analyses commenced from day 3 after harvest of GRS samples. Prior to all analytical tests, the shrimp were beheaded, peeled and deveined. In most cases, experimental/laboratory analyses were regularly implemented every two months during the 12-month period, which afforded between 5 and 7 data points of outcomes of storage measurements of tested parameters against storage time. Unless otherwise indicated, analyses were repeated three times and independently per treatment using different GRS samples. All chemicals and reagents employed are of analytical grade.
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