β-d-Fructofuranosidase, (FFase) (EC 3.2.1.26) which catalyzes the hydrolysis of terminal non-reducing β-d-fructofuranoside residue in sucrose, raffinose and related β-d-fructofuranosides, is produced by bacteria, fungi, higher plants, and some animals (Belcarz et al., 2002). However, Saccharomyces cerevisiae is the most preferred organism for FFase production due to its high sucrose-fermenting ability. FFase is widely used in food and beverage industry and one of the important applications of FFase is for production of non-crystallizable sugar (invert sugar) syrup from sucrose, which is used as a humectant in the manufacture of soft centered candies and fondants. FFase is also used whenever sucrose containing substrates are subjected to fermentation, viz. production of alcoholic beverages, lactic acid, glycerol and also in the manufacture of artificial honey and plasticizing agents. In addition to hydrolytic activity, FFase also exhibits transfructosylating activity at high sucrose concentrations, that may be used to obtain fructooligosaccharides (FOS), used as prebiotic formulations ( Emine et al., 2010).