CULTURE MEDIA AND INCUBATION TEMPERATURESMedia for the test may be prepared as described below or equivalent commercial media may beused provided that they comply with the requirements of the Growth Promotion Test of Aerobes,Anaerobes, and Fungi.The following culture media have been found to be suitable for the test for sterility. FluidThioglycollate Medium is primarily intended for the culture of anaerobic bacteria. However, it will alsodetect aerobic bacteria. Soybean–Casein Digest Medium is suitable for the culture of both fungi andaerobic bacteria.