The results found Water bottle amount of polymer that has been washed and left indefinitely after 4 days and 8 days are toxic to living organisms most experimental, with LC50 values of 24.54% and 24.20. %, respectively, of the survival of the species tested at 48 hours and, over time, after soaking in water for 16 days and 40 days LC50 values decrease is equal to 36.66% and 46.68%, respectively, which may be due. the polymer is then melted away in the early stages.