2.8.1.2. Milling and autoclaving pretreatment. Ten grams of
chipped and ground bagasse were put into a 250 mL Erlenmeyer flask then moistened with distilled water; the flasks were
steam-treated by autoclaving at 121 C and 1.5 bars for
20 min. Then extraction, filtration and determination of TRS
were carried out as previously mentioned.