Histopathological examination of burn injuries
Tissue specimens with dimensions of 0.5×0.5 cm were isolated from the burnt area at the midriff sections. The tissues were then kept in freshly prepared, neutral buffered solution with 10% formaldehyde for a period of 24 h. Next, staining using hematoxylin and eosin were performed on the sections and further analyzed under a light microscope (Nikon 516609; Nikon Corporation, Tokyo, Japan) at objective magnifications of ×20 and ×40.