Equal volumes of phenol–chloroform–isoamyl alcohol
were added (25:24:1), mixed well, allowed to settle for 1 min and then
centrifuged at 12,000 g for 10 min. Equal volumes of
chloroform isoamyl alcohol (24:1)were rapidly added to the resulting supernatant,
mixed well, allowed to settle for 1 min and then centrifuged at 12,000 g
for a further 10 min.
Equal volumes of phenol–chloroform–isoamyl alcoholwere added (25:24:1), mixed well, allowed to settle for 1 min and thencentrifuged at 12,000 g for 10 min. Equal volumes of chloroform isoamyl alcohol (24:1)were rapidly added to the resulting supernatant,mixed well, allowed to settle for 1 min and then centrifuged at 12,000 gfor a further 10 min.
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