2.2.3. Decolouration
The chitin residue was mixed with acetone at a solid/solvent ration of 1:10 (w/v) for 10 min, filtered, dried for 2 h at room
temperature, followed by bleaching with 0.315% NaOCl for 5 min at the same solid/solvent ration (No et al., 1995). The decoloured chitin was washed and filtered as described previously.