The LAB strains of marine origin Lb. curvatus BCS35 and E. faecium BNM58, previously isolated from dry-salted cod and albacore muscle,respectively (Gómez-Sala et al., 2015), were selected for the biopreservation studies of fresh fish. E. faecium BNM58 harbors the genetic determinants encoding enterocin L50, enterocin P and enterocin Q, while Lb. curvatus BCS35 contains sakacin P, sakacin X and sakacin Q (Gómez-Sala et al., 2015). The LAB strains were stored at −80 and −20 °C with 15% (v/v) of sterile glycerol as cryopreservative.