a) Wash any remaining larvae from the vessel into the sieve.
b) Clean the vessel with a sponge and hot detergent or bleach solution and rinse well.
c) Refill the vessel with appropriately treated seawater at the required temperature and salinity.
d) Grade the larvae by washing them through a stack of sieves of descending aperture size with filtered seawater. A guide to suitable mesh sizes for larvae of different shell lengths is given in Table 11.
e) Take small samples from each sieve upon which larvae have been retained and observe the appearance and activity of the larvae with the aid of a microscope. Discard any sieve fraction containing predominantly dead or slow-growing larvae.