Single PCR was performedusing DreamTaq PCRMasterMix (Fermentase),
which contains Taq polymerase, dNTPs, MgCl2 and the appropriate buffer. Each PCR tube contained 25 l reaction mixture
composed of 12.5 l of the master mix, 2.5 l of each forward
and reverse primer solution (in a final concentration of 200 nM),
3 l of DNA and nuclease-free water to complete the final volume.
PCR