The MFCs were inoculated with a solution of 1:1 buffer-substrate solution and inoculum where the inoculum was either unacclimated (primary clarifier effluent from the Penn State University’s Wastewater Treatment Plant) or pre-acclimated (combination of equal volumes of suspended mixed culture of bacteria from the MFCs fed with the pure single substrates at 1g/L for about 2 months: see Appendix A for MFC data).